Part:BBa_K4610001
Afu8g00510 (Fma-P450)
BBa_K4610001, Afu8g00510, encodes for the production of the protein Fma-P450 or Multifunctional cytochrome P450 monooxygenase af510/ Fumagillin biosynthesis cluster P450 monooxygenase. The protein produced, Fma-P450, is a necessary part of the fumagillin biosynthesis pathway.
After the initial substrate, farnesyl pyrophosphate (FPP) is converted to β-trans-bergamotene by Fma-TC, three sequential steps of oxidative transformations are catalyzed by Fma-P450. These three steps of oxidative transformations that are catalyzed by Fma-P450 first involve the hydroxylation of β-trans-bergamotene to yield 5R-hydroxyl-beta-trans-bergamotene. 5R-hydroxyl-beta-trans-bergamotene is then further oxidized to form 5-keto-cordycol. The third oxidation step produces 5-keto-demethoxy fumagillol through an additional epoxidation. The intermediate produced by the reaction involving Fma-P450, 5-keto-demethoxy fumagillol, continues the biosynthesis pathway to produce fumagillol in the first half of the biosynthesis pathway where it later evolves to form fumagillin, our target product. (Guruceaga et. al 2020)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 892
Illegal XhoI site found at 1475 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 988
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 312
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